I am Tan Zhi Yang, I completed my PhD in Biological Sciences in 2023. During my PhD, I was in Prof. Lu Gan’s Mitosis Lab in the Centre for BioImaging Sciences, National University of Singapore. There, I analysed budding and fission yeast using biochemical techniques and genetic modification, and used cryoelectron microscopy to study chromosome structure in situ.

In Prof. Liu’s Genomic Data Science Lab, I am setting up the wetlab to validate findings from genomic data. In particular, my first project involves using the CRISPR-Cas9 system with prime editing guide RNAs to introduce splicing quantitative trait loci (sQTL) variants into cell lines. This will verify whether they are causative of alternative splicing, and what the alternative splicing products are.

I enjoy travelling (when time permits).

Publications:

• Tan, Z. Y., Cai, S., Noble, A. J., Chen, J. K., Shi, J., & Gan, L. (2023). Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ. Elife, 12, RP87672.

• Cai, S., Chen, C., Tan, Z. Y., Huang, Y., Shi, J., & Gan, L. (2018). Cryo-ET reveals the macromolecular reorganization of S. pombe mitotic chromosomes in vivo. Proceedings of the National Academy of Sciences, 115(43), 10977-10982.

• Tan, Z. Y., Cai, S., Paithankar, S. A., Nie, X., Shi, J., & Gan, L. (2024). Macromolecular and cytological changes in fission yeast G0 nuclei. bioRxiv, 2020-01.